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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in several tumors, which leads to chromosome instability, and thus might lead to tumorigenesis. This protein was identified to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant job in advertising and marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are explained. Two added splice variants have been noted but specific nucleotide sequence facts just isn't nonetheless available.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide generally contains the epitope regarded via the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by itself, one can see which staining is precise.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins show unique expression and degradation patterns which add into the temporal coordination of each and every mitotic event. Cyclin E kinds a posh with and features to be a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and it is degraded as cells progress by means of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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